Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 97A1015

Format

BSA Free
View all D4-GDI/RhoGDI2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)BSA Free [NB100-56725]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)BSA Free [NB100-56725]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.
Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.
Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)BSA Free [NB100-56725]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)BSA Free [NB100-56725]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug antibody/test/1 million cells) and ((as seen in B). this is because (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells using 0.5 ug of NB100-56060. The shaded histogram represents cells without antibody.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free [NB100-56725]

Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated (right) Ramos cells using 0.5 ug of was used for this test.

Applications for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

0.5-1 ug/10^6 cells

Flow Cytometry

0.5-1 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-2 ug/ml

Western Blot

2 ug/ml
Application Notes
In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed.

Reviewed Applications

Read 1 review rated 4 using NB100-56725 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: D4-GDI/RhoGDI2

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Alternate Names

ARHGDIB Rho GDP dissociation inhibitor (GDI) beta, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2

Gene Symbol

ARHGDIB

Additional D4-GDI/RhoGDI2 Products

Product Documents for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

Certificate of Analysis

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Product Specific Notices for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

Customer Reviews for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free (1)

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  • D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT-29 human colon adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 07/18/2018
    HT-29 cells were treated with etoposide (100 ug/ml) for indicated time points. Total cell lysates were subjected to western blot. PVDF membrane were probed with 2 um/ml D4-GDI/RhoGDI2 (cleavage specific) Antibody (NB100-56725). A specific band was detected for cleaved RhoGDI2 at approximately 23 kDa. This experiment was conducted under reducing conditions.
    D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free NB100-56725

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