DAB2 Antibody (2H7C4) - BSA Free

Novus Biologicals | Catalog # NBP2-61884

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H7C4

Format

BSA Free
View all DAB2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human DAB2 (AA: 652-749) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAB2 Antibody (2H7C4) - BSA Free

Western Blot: DAB2 Antibody (2H7C4)BSA Free [NBP2-61884]

Western Blot: DAB2 Antibody (2H7C4)BSA Free [NBP2-61884]

Western Blot: DAB2 Antibody (2H7C4) [NBP2-61884] - Analysis using DOC2 mAb against human DOC2 (AA: 652-749) recombinant protein. (Expected MW is 36.6 kDa)
Immunocytochemistry/ Immunofluorescence: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

Immunocytochemistry/ Immunofluorescence: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

Immunocytochemistry/Immunofluorescence: DAB2 Antibody (2H7C4) [NBP2-61884] - Analysis of Hela cells using DOC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

Flow Cytometry: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

Flow Cytometry: DAB2 Antibody (2H7C4) [NBP2-61884] - Analysis of Hela cells using DOC2 mouse mAb (green) and negative control (red).
Western Blot: DAB2 Antibody (2H7C4)BSA Free [NBP2-61884]

Western Blot: DAB2 Antibody (2H7C4)BSA Free [NBP2-61884]

Western Blot: DAB2 Antibody (2H7C4) [NBP2-61884] - Analysis using DOC2 mAb against HEK293 (1) and DOC2 (AA: 652-749)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

ELISA: DAB2 Antibody (2H7C4) - BSA Free [NBP2-61884]

ELISA: DAB2 Antibody (2H7C4) [NBP2-61884] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for DAB2 Antibody (2H7C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAB2

This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.;

Long Name

Disabled Homolog 2, Mitogen-Responsive Phosphoprotein

Alternate Names

Disabled-2, DOC2

Gene Symbol

DAB2

Additional DAB2 Products

Product Documents for DAB2 Antibody (2H7C4) - BSA Free

Certificate of Analysis

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Product Specific Notices for DAB2 Antibody (2H7C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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