DAP12 Antibody

Novus Biologicals | Catalog # NBP1-52376

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bat, Canine, Equine, Monkey, Primate

Cited:

Human

Predicted:

Chimpanzee (100%), Rabbit (92%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Peptide ELISA, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Synthetic peptide C-KQRITETESPYQE from an internal region of human DAP12 (NP_003323.1; NP_937758.1).

Epitope

C-KQRITETESPYQE from an internal region

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (85%), Bovine (85%).

Specificity

Human DAP12. This antibody is expected to recognize both reported isoforms (NP_003323.1; NP_937758.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DAP12 Antibody

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376]

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376]

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376] - Analysis of anti-TYROBP / DAP12 antibody with human spleen at concentration 3 ug/ml.
Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376]

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376]

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376] - Analysis of anti-TYROBP / DAP12 antibody with human uterus at concentration 3 ug/ml.

Applications for DAP12 Antibody

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

3-6 ug/ml

Immunohistochemistry-Paraffin

3-6 ug/ml
Application Notes
IHC: NBP1-52376 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Use in FLOW reported in scientific literature (PMID: 31201275)..

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAP12

The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).

Long Name

DNAX-activation Protein 12

Alternate Names

KARAP, TYROBP

Entrez Gene IDs

7305 (Human)

Gene Symbol

TYROBP

UniProt

O43914 (Human)

Additional DAP12 Products

Product Documents for DAP12 Antibody

Certificate of Analysis

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Product Specific Notices for DAP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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