DAPK3/ZIPK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76962

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DAPK3/ZIPK Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK. Amino Acid Squence: RRRNVRGEDSGRKPERRRLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAPK3/ZIPK Antibody - BSA Free

Western Blot: DAPK3/ZIPK AntibodyBSA Free [NBP1-76962]

Western Blot: DAPK3/ZIPK AntibodyBSA Free [NBP1-76962]

Western Blot: DAPK3/ZIPK Antibody [NBP1-76962] - Analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] -

Immunocytochemistry/Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Staining of ZIPK in Jurkat cells with ZIPK antibody at 5 ug/ml.
DAPK3/ZIPK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody - BSA Free [NBP1-76962] -

Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody - BSA Free [NBP1-76962] - Immunofluorescence of DAPK3/ZIPK in Jurkat cells with DAPK3/ZIPK antibody at 10 ug/mL.

Applications for DAPK3/ZIPK Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Western Blot

1 ug/ml
Application Notes
An approximate 40 kDa band can be detected in Western Blot, which represents the pro-enzyme of DNase II.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAPK3/ZIPK

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

Long Name

Death-associated Protein Kinase 3

Alternate Names

Dlk, ZIPK

Gene Symbol

DAPK3

UniProt

BAA81746 (Human)

Additional DAPK3/ZIPK Products

Product Documents for DAPK3/ZIPK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DAPK3/ZIPK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for DAPK3/ZIPK Antibody - BSA Free

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Protocols

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