DAX1/NR0B1 Antibody (OTI5F5)
Novus Biologicals | Catalog # NBP2-46212
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI5F5
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 161-430 of human NR0B1 (NP_000466) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DAX1/NR0B1 Antibody (OTI5F5)
![Western Blot: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]](https://resources.rndsystems.com/images/products/DAX1-NR0B1-Antibody-5F5-Western-Blot-NBP2-46212-img0002.jpg "Western Blot: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]")
Western Blot: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]
Western Blot: DAX1/NR0B1 Antibody (5F5) [NBP2-46212] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B1.![Immunohistochemistry: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]](https://resources.rndsystems.com/images/products/DAX1-NR0B1-Antibody-5F5-Immunohistochemistry-NBP2-46212-img0001.jpg "Immunohistochemistry: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]")
Immunohistochemistry: DAX1/NR0B1 Antibody (OTI5F5) [NBP2-46212]
Immunohistochemistry: DAX1/NR0B1 Antibody (5F5) [NBP2-46212] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for DAX1/NR0B1 Antibody (OTI5F5)
Application
Recommended Usage
Immunohistochemistry
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DAX1/NR0B1
Long Name
Orphan Nuclear Receptor DAX1/Nuclear Receptor Subfamily 0, Group B, Member 1
Alternate Names
AHC, AHCH, NR0B1
Gene Symbol
NR0B1
Additional DAX1/NR0B1 Products
Product Documents for DAX1/NR0B1 Antibody (OTI5F5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DAX1/NR0B1 Antibody (OTI5F5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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