Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD209) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN, CD209L or CD299) are type II transmembrane proteins that are mannose-specific, Calcium-dependent (C-type) lectins.
DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-52649
Recombinant Monoclonal Antibody
![Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7)ChimericAzide and BSA Free [NBP2-52649]](https://resources.rndsystems.com/images/products/DC-SIGNR-CD299-CLEC4M-Antibody-16E7-Chimeric-Western-Blot-NBP2-52649-img0002.jpg "Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7)ChimericAzide and BSA Free [NBP2-52649]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 16E7
Format
Azide and BSA Free
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Product Specifications
Immunogen
CD299
Specificity
Human CD299, which is an oligomeric type II transmembrane protein with a C-type lectin extracellular domain, the expression of which is restricted to immature DC, macrophages in the lung, and endothelial cells in the liver. It binds ICAM-3 and ICAM-7 to mediate the interaction of DC with T lymphocytes and endothelial cells in the initial stages of immune response and in the migratory behavior of DC. CD299 also binds the gp120 protein of HIV and the E2 envelope protein of HCV, thereby playing a role in viral infection.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7)ChimericAzide and BSA Free [NBP2-52649]
Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric [NBP2-52649] - Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (NBP2-52649) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. NBP2-52649 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.
Immunocytochemistry /Immunofluorescence: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free [NBP2-52649] -
Immunocytochemistry /Immunofluorescence: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free [NBP2-52649] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-52649 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52649, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.Applications for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: DC-SIGNR/CD299
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
Alternate Names
CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN
Gene Symbol
CLEC4M
Additional DC-SIGNR/CD299 Products
Product Documents for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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