DCUN1D5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-30432
![Immunocytochemistry/ Immunofluorescence: DCUN1D5 Antibody [NBP2-30432]](https://resources.rndsystems.com/images/products/DCUN1D5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30432-img0005.jpg "Immunocytochemistry/ Immunofluorescence: DCUN1D5 Antibody [NBP2-30432]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (91%), Rat (93%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSKKCLAWF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DCUN1D5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: DCUN1D5 Antibody [NBP2-30432]
Immunocytochemistry/Immunofluorescence: DCUN1D5 Antibody [NBP2-30432] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & nucleoli.![Immunohistochemistry: DCUN1D5 Antibody [NBP2-30432]](https://resources.rndsystems.com/images/products/DCUN1D5-Antibody-Immunohistochemistry-NBP2-30432-img0001.jpg "Immunohistochemistry: DCUN1D5 Antibody [NBP2-30432]")
Immunohistochemistry: DCUN1D5 Antibody [NBP2-30432]
Immunohistochemistry: DCUN1D5 Antibody [NBP2-30432] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.![Simple Western: DCUN1D5 Antibody [NBP2-30432]](https://resources.rndsystems.com/images/products/DCUN1D5-Antibody-Simple-Western-NBP2-30432-img0003.jpg "Simple Western: DCUN1D5 Antibody [NBP2-30432]")
Simple Western: DCUN1D5 Antibody [NBP2-30432]
Simple Western: DCUN1D5 Antibody [NBP2-30432] - Simple Western lane view shows a specific band for DCUN1D5 in 0.2 mg/ml of SW480 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.![Simple Western: DCUN1D5 Antibody [NBP2-30432]](https://resources.rndsystems.com/images/products/DCUN1D5-Antibody-Simple-Western-NBP2-30432-img0004.jpg "Simple Western: DCUN1D5 Antibody [NBP2-30432]")
Simple Western: DCUN1D5 Antibody [NBP2-30432]
Simple Western: DCUN1D5 Antibody [NBP2-30432] - Electropherogram image of the corresponding Simple Western lane view. DCUN1D5 antibody was used at 1:10 dilution on SW480 lysate(s) respectively.Applications for DCUN1D5 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in SW480 lysates, separated by Size, antibody dilution of 1:10
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DCUN1D5
Alternate Names
DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae), DCN1-like protein 5, DCUN1 domain-containing protein 5, Defective in cullin neddylation protein 1-like protein 5, FLJ32431, FLJ37425, MGC2714
Gene Symbol
DCUN1D5
UniProt
Additional DCUN1D5 Products
Product Documents for DCUN1D5 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DCUN1D5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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