DDOST Antibody (OTI2B4)

Novus Biologicals | Catalog # NBP2-46439

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B4
View all DDOST Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDOST Antibody (OTI2B4)

Western Blot: DDOST Antibody (OTI2B4) [NBP2-46439]

Western Blot: DDOST Antibody (OTI2B4) [NBP2-46439]

Western Blot: DDOST Antibody (2B4) [NBP2-46439] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST.
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (OTI2B4) [NBP2-46439]

Immunohistochemistry: DDOST Antibody (2B4) [NBP2-46439] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for DDOST Antibody (OTI2B4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DDOST

DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq]

Alternate Names

advanced glycation endproduct receptor 1, DDOST 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, dolichyl-diphosphooligosaccharide-protein glycotransferase, EC 2.4.1.119, KIAA0115MGC2191, Oligosaccharyl transferase 48 kDa subunit, oligosaccharyltransferase 48 kDa subunit, OST48AGER1, OSTOKSWcl45, WBP1

Gene Symbol

DDOST

Additional DDOST Products

Product Documents for DDOST Antibody (OTI2B4)

Certificate of Analysis

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Product Specific Notices for DDOST Antibody (OTI2B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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