DDX17 Antibody - BSA Free
Novus Biologicals | Catalog # NB200-352
![Western Blot: DDX17 Antibody [NB200-352]](https://resources.rndsystems.com/images/products/DDX17-Antibody-Western-Blot-NB200-352-img0009.jpg "Western Blot: DDX17 Antibody [NB200-352]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DDX17 Antibody - BSA Free
Western Blot: DDX17 Antibody [NB200-352]
Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.![Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352]](https://resources.rndsystems.com/images/products/DDX17-Antibody-Immunohistochemistry-Paraffin-NB200-352-img0008.jpg "Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352]")
Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352]
Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Western Blot: DDX17 Antibody [NB200-352]](https://resources.rndsystems.com/images/products/DDX17-Antibody-Western-Blot-NB200-352-img0005.jpg "Western Blot: DDX17 Antibody [NB200-352]")
Western Blot: DDX17 Antibody [NB200-352]
Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibodyApplications for DDX17 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 -1:2000
Immunohistochemistry-Paraffin
1:500 -1:2000
Western Blot
1:1000 - 1:15000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DDX17
Long Name
DEAD Box Protein 17
Alternate Names
p72, RH70
Entrez Gene IDs
10521 (Human)
Gene Symbol
DDX17
UniProt
Additional DDX17 Products
Product Documents for DDX17 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DDX17 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for DDX17 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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