DDX19B Antibody

Western Blot: DDX19B Antibody [NB100-760]

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Western Blot: DDX19B Antibody [NB100-760]

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Western Blot: DDX19B Antibody [NB100-760]

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Western Blot: DDX19B Antibody [NB100-760]

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Chromatin Immunoprecipitation: DDX19B Antibody [NB100-760] -

Chromatin Immunoprecipitation: DDX19B Antibody [NB100-760] - MKL1 contains two Ddx19 interaction sites.(a) Co-IPs of endogenous MKL1 with endogenous Ddx19. NIH 3T3 cytoplasmic extract was immunoprecipitated with anti-Ddx19 antibody & the immunoprecipitates were blotted for indicated antibodies. Beads without antibody (no ab) were used as a negative control. (b) HA-tagged MKL1 constructs were immunoprecipitated from NIH 3T3 lysates & their expression was detected by HA-antibody. Co-precipitated endogenous Ddx19 was detected by western blotting (WB). Empty HA was used as a negative control. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - Ddx19 interacts with MKL1 RPEL domain & Ipo beta.(a) Ddx19 recruitment from Hela cell lysate by GST-RPEL & GST-Ipo beta (left). WB, western blotting; (−), without Ran-Q69L; (+), with RanQ69L. Corresponding GST baits are stained with Ponceau to ensure the equal loading of the samples (below). Input sample corresponds to 5% of the Hela cell lysate used in the assay. (b) GST-RPEL was used as a bait for pull down of Ddx19 & Ipo beta from the Hela cell lysate in the presence of increasing amounts of LatB-actin (0.25–10 μM) (left). Arrow indicates the increasing amounts of actin in the corresponding Ponceau staining (below). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms6978), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - The accumulation of viral proteins & RNAs is reduced in DDX19-depleted cells.A549 cells were treated with control (C) or DDX19 (19) siRNAs & infected with WSN (5 pfu/cell). (a) Total extracts were prepared at the indicated times post-infection & analyzed by immunoblots using antibodies directed against the indicated proteins. Results representative of 3 independent experiments are shown. Cropped blots are shown. The corresponding full-length blots are shown in Figure S3. (b–e) The levels of NP or NA mRNAs & vRNAs (b or c & d or e, respectively) were determined at the indicated times post-infection by strand specific RT-qPCR & were normalized to the level of the same RNA species at 3 hpi in cells treated with the control siRNAs. The results are expressed as the mean ± SEM of three independent experiments & the significance was tested with a one-sample t test using GraphPad Prism Software (*p < 0.05; **p < 0.01; ***p < 0.001). Dashed lines were used to indicate that the Y-axes have been segmented. Different scales were used for the mRNA & vRNA graphs. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DDX19B Antibody [NB100-760] -

Western Blot: DDX19B Antibody [NB100-760] - The earliest steps of IAV replication are not affected by DDX19 depletion.A549 cells treated with control (C in a & b, dark grey bars in c & d) or DDX19 (19 in a & b, light grey bars in c & d) siRNAs were infected with WSN (5 pfu/cell). (a) Total extracts from control cells treated with CHX (+CHX) or not (−CHX) were prepared at 6 hpi & analyzed by immunoblots using antibodies directed against the indicated proteins. Cropped blots are shown. The corresponding full-length blots are shown in Figure S4. (b) Cytoplasmic & nuclear fractions were prepared at 4 hpi. Aliquots of the indicated subcellular fractions were analyzed by immunoblots with antibodies directed against MEK1/2 kinase (cytoplasmic marker), TBP (nuclear marker) & NP. Alternatively, total RNAs were extracted & the levels of GAPDH pre-mRNA, a nuclear marker, were determined by real-time RT-PCR. Results are expressed as the mean of two determinations of the crossing point value (Cp). Cropped blots are shown. The corresponding full-length blots are shown in Figure S5. (c,d) Infection was carried out for 6 h in the presence of CHX (100 µg/mL). Total RNAs were isolated from cytoplasmic (solid bars) & nuclear (hatched bars) fractions, & the levels of NP & NA vRNAs were determined by strand specific RT-qPCR. The results are expressed as the mean percentages ± SEM of cytoplasmic & nuclear vRNAs levels determined in three independent experiments (c). Total RNAs were extracted & the levels of NP or NA mRNAs & vRNAs were determined by strand specific RT-qPCR. The results are expressed as the mean ratios of mRNA/vRNA ± SEM determined in three independent experiments (d). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep33763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.