DDX3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32780

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%), Primate (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DDX3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (97%).

Localization

Cytoplasm, Nucleus

Specificity

This antibody recognizes both DDX3Y and DDX3X.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DDX3 Antibody - BSA Free

Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP1-32780]

Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP1-32780]

Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-32780] - Analysis of paraformaldehyde-fixed HeLa, using DDX3Y antibody at 1:200 dilution.
Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780]

Western Blot: DDX3 Antibody [NBP1-32780] - Sample (30 ug of whole cell lysate)A: H1299B: Hep G2 7. 5% SDS PAGE, antibody diluted at 1:10000.
DDX3 Antibody

Western Blot: DDX3 Antibody [NBP1-32780] -

Western Blot: DDX3 Antibody [NBP1-32780] - DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 50 ug rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (NBP1-32780) dilution: 1:2000
DDX3 Antibody

Western Blot: DDX3 Antibody [NBP1-32780] -

Western Blot: DDX3 Antibody [NBP1-32780] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (NBP1-32780) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DDX3 Antibody

Western Blot: DDX3 Antibody [NBP1-32780] -

Western Blot: DDX3 Antibody [NBP1-32780] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (NBP1-32780) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for DDX3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DDX3

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.

Alternate Names

DBXCAP-Rf, DDX14, DDX3DEAD/H box-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD box, X isoform, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, EC 3.6.1, EC 3.6.4.13, helicase like protein 2, Helicase-like protein 2, HLP2ATP-dependent RNA helicase DDX3X

Gene Symbol

DDX3X

Additional DDX3 Products

Product Documents for DDX3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DDX3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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