DEC-205/CD205 Antibody (JB87-35)
Novus Biologicals | Catalog # NBP2-75467
Recombinant Monoclonal Antibody
![Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]](https://resources.rndsystems.com/images/products/DEC-205-CD205-Antibody-JB87-35-Western-Blot-NBP2-75467-img0005.jpg "Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JB87-35 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human DEC-205/CD205 aa 1,560-1,722 / 1,722. (SwissProt: O60449 Human; SwissProt: Q60767 Mouse)
Localization
Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
198 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DEC-205/CD205 Antibody (JB87-35)
Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]
Western Blot: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of LY75 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]](https://resources.rndsystems.com/images/products/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0004.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]")
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded mouse colon tissue using anti-LY75 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]](https://resources.rndsystems.com/images/products/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0001.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]")
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human tonsil tissue using anti-LY75 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]](https://resources.rndsystems.com/images/products/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0002.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]")
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human colon cancer tissue using anti-LY75 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]](https://resources.rndsystems.com/images/products/DEC-205-CD205-Antibody-JB87-35-Immunohistochemistry-Paraffin-NBP2-75467-img0003.jpg "Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]")
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467]
Immunohistochemistry-Paraffin: DEC-205/CD205 Antibody (JB87-35) [NBP2-75467] - Analysis of paraffin-embedded human kidney tissue using anti-LY75 antibody. Counter stained with hematoxylin.Applications for DEC-205/CD205 Antibody (JB87-35)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DEC-205/CD205
Alternate Names
CD205, DEC205, Ly75
Gene Symbol
LY75
Additional DEC-205/CD205 Products
Product Documents for DEC-205/CD205 Antibody (JB87-35)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DEC-205/CD205 Antibody (JB87-35)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
