Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 3D1

Format

Azide and BSA Free
View all Dectin-2/CLEC6A Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 75-139 of human Dectin-2 was used as the immunogen for the antibody. 90-106

Reactivity Notes

Orangutan, Chimpanzee (100%), Rhesus monkey, Bovine, Sheep (83%), Mouse (65%), Rat (52%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

Western Blot: Dectin-2/CLEC6A Antibody (3D1)Azide and BSA Free [NBP2-36486]

Western Blot: Dectin-2/CLEC6A Antibody (3D1)Azide and BSA Free [NBP2-36486]

Western Blot: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Analysis of Dectin-2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart lysate, 4) rat heart lysate and 5) human Ramos cell lysate using Dectin-2 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunohistochemistry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Immunohistochemistry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Immunohistochemistry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Normal human spleen tissue stained with Dectin-2 antibody (5 ug/mL), peroxidase-conjugate and DAB chromogen. Human tissue TMA was used for this test. Image from the standard format of this antibody.
Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - An intracellular stain was performed on THP-1 cells with Dectin-2/CLEC6A (3D1) antibody NBP2-27159AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free [NBP2-36486]

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Surface staining of Dectin-2 on 5x10^5 human PBMCs using 0.5 ug of Dectin-2 antibody (red) and 0.5 ug of isotype control antibody (green). Image from the standard format of this antibody.

Applications for Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dectin-2/CLEC6A

Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcR 7; for internalization and to activate NF-kB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level.

Alternate Names

CLEC6A, CLECSF10, Dectin2

Gene Symbol

CLEC6A

Additional Dectin-2/CLEC6A Products

Product Documents for Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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