Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-85474

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # AP-MAB0801

Format

Azide and BSA Free
View all Dendritic Cell Marker Primary Antibodies »

Product Specifications

Immunogen

Mouse Dendritic cells

Marker

Dendritic Cell Marker

Specificity

Reacts with mouse dendritic cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse DCs using NB110-85474 (x 40).
Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - 4% PFA fixed and paraffin embedded mouse Liver and Lung sections from LPS treated mice was subjected to staining (DAB) of DC marker.
Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free [NB110-85474]

Immunohistochemistry-Paraffin: Dendritic Cell Marker Antibody (AP-MAB0801) [NB110-85474] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of mouse DCs using NB110-85474 (x 20).

Applications for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:1000

Immunohistochemistry-Paraffin

1:25-1:50
Application Notes
The enzymatic antigen retrieval method (proteinase K digestion) was used in IHC-P that led to the generation of the images posted on this site. This antibody is CyTOF ready.

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dendritic Cell Marker

Dendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.

Alternate Names

DCs, Dendritic cell

Additional Dendritic Cell Marker Products

Product Documents for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Dendritic Cell Marker Antibody (AP-MAB0801) - Azide and BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Could you let us know which kind of dendritic cells from mouse have been used to generate the antibody? We want to use the antibody for staining of intravascular dendritische cells of the central nervous system.

    A: The lab has informed me that purified Spleen dendritic cells were used as immunogen for this clone. The lab also informed me that the immunogen cells were DC enriched cells from spleen cell suspension and for antibody binding 100% pure cells are not required.

  • Q: Does the antibody stain DCs in mouse spleen sections?

    A: Yes

  • Q: I have a question regarding the antibody AP-MAB0801: The product description is very unspecific, saying it recognizes a surface marker of mouse DCs. We would like to know which surface marker this antibody recognizes, so we know which subsets of DCs will be stained.

    A: This clone detects the Dendritic Cell Inhibitory Receptor 2 on mouse DC cells.

  • Q: Which DCs does it stain?

    A: NB110-85474 will stain all mouse dendridic cells.

  • Q: Could you let us know which kind of dendritic cells from mouse have been used to generate the antibody? We want to use the antibody for staining of intravascular dendritische cells of the central nervous system.

    A: The lab has informed me that purified Spleen dendritic cells were used as immunogen for this clone. The lab also informed me that the immunogen cells were DC enriched cells from spleen cell suspension and for antibody binding 100% pure cells are not required.

  • Q: Does the antibody stain DCs in mouse spleen sections?

    A: Yes

  • Q: I have a question regarding the antibody AP-MAB0801: The product description is very unspecific, saying it recognizes a surface marker of mouse DCs. We would like to know which surface marker this antibody recognizes, so we know which subsets of DCs will be stained.

    A: This clone detects the Dendritic Cell Inhibitory Receptor 2 on mouse DC cells.

  • Q: Which DCs does it stain?

    A: NB110-85474 will stain all mouse dendridic cells.

  • Q: Could you let us know which kind of dendritic cells from mouse have been used to generate the antibody? We want to use the antibody for staining of intravascular dendritische cells of the central nervous system.

    A: The lab has informed me that purified Spleen dendritic cells were used as immunogen for this clone. The lab also informed me that the immunogen cells were DC enriched cells from spleen cell suspension and for antibody binding 100% pure cells are not required.

  • Q: Does the antibody stain DCs in mouse spleen sections?

    A: Yes

  • Q: I have a question regarding the antibody AP-MAB0801: The product description is very unspecific, saying it recognizes a surface marker of mouse DCs. We would like to know which surface marker this antibody recognizes, so we know which subsets of DCs will be stained.

    A: This clone detects the Dendritic Cell Inhibitory Receptor 2 on mouse DC cells.

  • Q: Which DCs does it stain?

    A: NB110-85474 will stain all mouse dendridic cells.

  • Q: Could you let us know which kind of dendritic cells from mouse have been used to generate the antibody? We want to use the antibody for staining of intravascular dendritische cells of the central nervous system.

    A: The lab has informed me that purified Spleen dendritic cells were used as immunogen for this clone. The lab also informed me that the immunogen cells were DC enriched cells from spleen cell suspension and for antibody binding 100% pure cells are not required.

  • Q: Does the antibody stain DCs in mouse spleen sections?

    A: Yes

  • Q: I have a question regarding the antibody AP-MAB0801: The product description is very unspecific, saying it recognizes a surface marker of mouse DCs. We would like to know which surface marker this antibody recognizes, so we know which subsets of DCs will be stained.

    A: This clone detects the Dendritic Cell Inhibitory Receptor 2 on mouse DC cells.

  • Q: Which DCs does it stain?

    A: NB110-85474 will stain all mouse dendridic cells.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for Antibodies