Dengue Virus NS5 Antibody

Novus Biologicals | Catalog # NBP2-42900

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Porcine, Virus

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Dengue Virus NS5 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue Virus NS5 (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Reactivity Notes

Reactive to Dengue Virus. Reactivity to the Zika Virus reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dengue Virus NS5 Antibody

Western Blot: Dengue Virus NS5 Antibody [NBP2-42900]

Western Blot: Dengue Virus NS5 Antibody [NBP2-42900]

Western Blot: NS5 Antibody [NBP2-42900] - Analysis of A. 30 ug BHK-21 whole cell extract B. 30 ug whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 ug whole cell extract of Dengue virus type 3 infected BHK-21 cells 7.5 % SDS-PAGE NS5 (Dengue virus) antibody dilution: 1:5000
Immunocytochemistry/ Immunofluorescence: Dengue Virus NS5 Antibody [NBP2-42900]

Immunocytochemistry/ Immunofluorescence: Dengue Virus NS5 Antibody [NBP2-42900]

Immunocytochemistry/Immunofluorescence: NS5 Antibody [NBP2-42900] - Analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody at 1:2000 dilution.
Immunohistochemistry-Paraffin: Dengue Virus NS5 Antibody [NBP2-42900]

Immunohistochemistry-Paraffin: Dengue Virus NS5 Antibody [NBP2-42900]

Immunohistochemistry-Paraffin: NS5 Antibody [NBP2-42900] - Zika-infected prostate organoids. Organoids composed of 19I mesenchymal stem cells and LNCaP prostate epithelial cells. Prostate organoids were infected with Zika virus isolate HN16 at an MOI of 1. Organoids were processed and stained for NS5 at 4 days post-infection. Image from verified customer review.
Dengue Virus NS5 Antibody

Western Blot: Dengue Virus NS5 Antibody [NBP2-42900] -

Non-infected (-) and infected (+) C6/36 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (NBP2-42900) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Dengue Virus NS5 Antibody

Immunocytochemistry/ Immunofluorescence: Dengue Virus NS5 Antibody [NBP2-42900] -

Dengue virus NS5 protein antibody detects Dengue virus NS5 protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dengue virus NS5 protein stained by Dengue virus NS5 protein antibody (NBP2-42900) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for Dengue Virus NS5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry-Paraffin

Validated by verified customer review.

Western Blot

1:1000 - 1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-42900 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dengue Virus NS5

Dengue virus is a single -stranded, positive-sense RNA virus belonging to the Flavivirdae family and is the causative agent of Dengue fever (1-3). Other viruses in the same family include Japanese encephalitis virus, West Nile virus, and yellow fever (2,3). The Dengue virus is primarily transmitted through Aedes mosquito bites, with 100-400 million people infected annually and approximately 20,000 deaths worldwide per year (1,3). There are four known Dengue virus serotypes (DENV 1-4) that share 65% amino acid sequence similarity (1-2). The serotypes can be further classified into genotypes according to geographical distribution (2,3). The structure of the Dengue virus is ~50 nanometers (nm) in diameter, spherical in shape, and consists of an enveloped single-stranded RNA, an icosahedral nucleocapsid core, and a lipid bilayer (1-3). The Dengue virus genome is 11-kilobases (kb) long and has a 5' untranslated region (UTR), an open reading frame (ORF), and a 3' UTR (1,3). The ORF encodes a polyprotein for the translation of the three structural proteins and seven non-structural proteins (1-3). The structural proteins are the capsid (100 amino acids (aa), 12 kilodalton (kDa)), the pre-membrane (166 aa) or membrane (75 aa, 8.2-8.5 kDa), and envelope (495 aa, 53 kDa) (1-3). The nonstructural proteins are NS1 (350 aa, 45 kDa), NS2A (218 aa, 22 kDa), NS2B (130 aa, 14 kDa), NS3 (618 aa, 70 kDa), NS4A (150 aa, 16 kDa), NS4B (245-249 aa, 27 kDa), NS5 (900 aa, 104 kDa) (1-3).

Dengue virus entry into host cells occurs via receptor-mediated endocytosis by receptor molecules including the mannose receptor, heparan sulfate, glycosaminoglycans, and DC-SIGN (1,3). Following attachment, the virus is endocytosed in clathrin-coated vesicles (1,3). Following internalization, clathrin disassembles and endosomal processing occurs, allowing viral fusion, disassembly, and release of viral RNA (1,3). This release results in viral translation and replication, virus assembly and maturation, and eventual exocytosis of the mature virus (1,3). Infection can result in a wide range of clinical symptoms including mild disease such as Dengue fever which is characterized by fever, headache, joint pain, rash, and retro-orbital pain, or severe, life-threatening conditions like Dengue hemorrhagic fever or Dengue shock syndrome which involves vascular permeability and leakage (1-3). Host immune response against infection includes innate immune response via interferon secretion and pro-inflammatory cytokine production, as well as adaptive immune response involving cellular and humoral components like T cell activation and B-cell mediated antibody production (1-3). As far as treatment for Dengue virus infection, there no commercial antiviral agents, though some anti-pyretics and certain phenolic compounds do show promise in treating infection (1-3). However, Resveratol, an antiviral for other Flavivirus, has been shown to directly attack the Dengue virus genome (1). While more work needs to be done, there are some live-attenuated tetravalent Dengue virus vaccine candidates in clinal trials including DENVax and TV003/TV005 (1-3).

References

1. Nanaware N, Banerjee A, Mullick Bagchi S, Bagchi P, Mukherjee A. Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses. Viruses. 2021;13(10):1967. Published 2021 Sep 30. https://doi.org/10.3390/v13101967

2. Harapan H, Michie A, Sasmono RT, Imrie A. Dengue: A Minireview. Viruses. 2020;12(8):829. Published 2020 Jul 30. https://doi.org/10.3390/v12080829

3. Roy SK, Bhattacharjee S. Dengue virus: epidemiology, biology, and disease aetiology. Can J Microbiol. 2021;67(10):687-702. https://doi.org/10.1139/cjm-2020-0572

Alternate Names

Dengue NS5, Dengue NS5 protein

Gene Symbol

POLY

Additional Dengue Virus NS5 Products

Product Documents for Dengue Virus NS5 Antibody

Certificate of Analysis

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Product Specific Notices for Dengue Virus NS5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Dengue Virus NS5 Antibody

Customer Reviews for Dengue Virus NS5 Antibody (1)

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Customer Images

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  • NS5 Antibody
    Name: Jennifer Spencer
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Prostate organoids
    Species: Human
    Verified Customer | Posted 08/02/2018
    IHC on Zika-infected prostate organoids. Organoids composed of 19I mesenchymal stem cells and LNCaP prostate epithelial cells. Prostate organoids were infected with Zika virus isolate HN16 at an MOI of 1.
    Organoids were processed and stained for NS5 at 4 days post-infection.
    Dengue Virus NS5 Antibody NBP2-42900

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