DENN/MADD Domain Containing 2D Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-30995
![Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]](https://resources.rndsystems.com/images/products/DENN-MADD-Domain-Containing-2D-Antibody-Western-Blot-NBP2-30995-img0002.jpg "Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PPQDNSGEALKEPERAQEHSLPNFAGGQHFFEYLLVVSLKKKRSEDDYEPIITYQFPKRENLLRGQQEEEERLLKAI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DENN/MADD Domain Containing 2D Antibody - BSA Free
Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]
Western Blot: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MOLT-4![Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]](https://resources.rndsystems.com/images/products/DENN-MADD-Domain-Containing-2D-Antibody-Immunohistochemistry-NBP2-30995-img0001.jpg "Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]")
Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]
Immunohistochemistry: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelium.![Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]](https://resources.rndsystems.com/images/products/DENN-MADD-Domain-Containing-2D-Antibody-Simple-Western-NBP2-30995-img0003.jpg "Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]")
Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]
Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Simple Western lane view shows a specific band for DENN/MADD Domain Containing 2D in 0.2 mg/ml of MOLT-4 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]](https://resources.rndsystems.com/images/products/DENN-MADD-Domain-Containing-2D-Antibody-Simple-Western-NBP2-30995-img0004.jpg "Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]")
Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995]
Simple Western: DENN/MADD Domain Containing 2D Antibody [NBP2-30995] - Electropherogram image of the corresponding Simple Western lane view. DENN/MADD Domain Containing 2D antibody was used at 1:25 dilution on MOLT-4 lysate(s) respectively.Applications for DENN/MADD Domain Containing 2D Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Simple Western
1:25
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
See Simple Western Antibody Database for Simple Western validation: Tested in MOLT-4 lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 54 kDa
See Simple Western Antibody Database for Simple Western validation: Tested in MOLT-4 lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 54 kDa
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DENN/MADD Domain Containing 2D
Alternate Names
DENN Domain-Containing Protein 2D, DENND2D, RP5-1180E21.2
Gene Symbol
DENND2D
UniProt
Additional DENN/MADD Domain Containing 2D Products
Product Documents for DENN/MADD Domain Containing 2D Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DENN/MADD Domain Containing 2D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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