DePsipher Assay

Label: Lipophilic cation
Testing Format: Flow Cytometry, Fluorescence Microscopy
Sample Type: Unfixed cells
Size: 100 Tests
DePsipher Assay (Catalog # 6300-100-K)
Identification of Apoptotic INT407 Cells using the DePsipher Kit. NT407 human embryonic intestinal cells were treated with 25 µM etoposide for 8 hours and then incubated with the DePsipher reagent in reaction buffer for 30 minutes (both provided in the DePsipher Kit, Catalog # 6300-100-K). Healthy cells (containing red aggregates) were differentiated from apoptotic cells (containing only green monomers) by fluorescence microscopy.

Disruption of the electrochemical gradient across the mitochondrial transmembrane is one of the early intracellular events to occur following induction of apoptosis. The DePsipher™ kit uses a lipophilic cation to identify changes in the potential of the mitochondrial membrane.



In normal, healthy cells, the DePsipher reagent aggregates in the mitochondria to form an orange fluorescent compound. However, in apoptotic cells, when the mitochondrial membrane potential is disrupted, the DePsipher reagent remains in its monomeric form and fluoresces green. These changes in fluorescence can be monitored either by microscopy or flow cytometry.

 

DePsipher is a trademark of Trevigen, Inc.

Preparation and Storage
  • Stability & Storage
    The product requires storage at -20 to -70 °C and 2 - 8 °C.  Consult the product insert for specific storage temperatures. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Sample Type
  1. Potentiation of LPS-Induced Apoptotic Cell Death in Human Hepatoma HepG2 Cells by Aspirin via ROS and Mitochondrial Dysfunction: Protection by N-Acetyl Cysteine
    PLoS ONE, 2016;11(7):e0159750. 2016
  2. Inhibition of mTOR-dependent autophagy sensitizes leukemic cells to cytarabine-induced apoptotic death.
    Authors: Bosnjak M, Ristic B, Arsikin K, Mircic A, Suzin-Zivkovic V, Perovic V, Bogdanovic A, Paunovic V, Markovic I, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L
    PLoS ONE, 2014;9(4):e94374. 2014
  3. NAC attenuates LPS-induced toxicity in aspirin-sensitized mouse macrophages via suppression of oxidative stress and mitochondrial dysfunction.
    Authors: Raza H, John A, Shafarin J
    PLoS ONE, 2014;9(7):e103379. 2014
  4. Idarubicin induces mTOR-dependent cytotoxic autophagy in leukemic cells.
    Authors: Ristic B, Bosnjak M, Arsikin K, Mircic A, Suzin-Zivkovic V, Bogdanovic A, Perovic V, Martinovic T, Kravic-Stevovic T, Bumbasirevic V, Trajkovic V, Harhaji-Trajkovic L
    Exp Cell Res, 2014;326(1):90-102. 2014
  5. Obesity is associated with increased seminal insulin and leptin alongside reduced fertility parameters in a controlled male cohort.
    Authors: Leisegang K, Bouic P, Menkveld R, Henkel R
    Reprod Biol Endocrinol, 2014;12(0):34. 2014
  6. Estrogenic compounds are not always cardioprotective and can be lethal in males with genetic heart disease.
    Authors: Haines, Christop, Harvey, Pamela A, Luczak, Elizabet, Barthel, Kristen, Konhilas, John P, Watson, Peter A, Stauffer, Brian L, Leinwand, Leslie A
    Endocrinology, 2012;153(9):4470-9. 2012
  7. Implications of altered glutathione metabolism in aspirin-induced oxidative stress and mitochondrial dysfunction in HepG2 cells.
    Authors: Raza H, John A,
    PLoS ONE, 2012;7(4):e36325. 2012
  8. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011;39(4):771-9. 2011
  9. Opposite effects of nanocrystalline fullerene (C(60)) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C(60).
    Authors: Zogovic NS, Nikolic NS, Vranjes-Djuric SD, Harhaji LM, Vucicevic LM, Janjetovic KD, Misirkic MS, Todorovic-Markovic BM, Markovic ZM, Milonjic SK, Trajkovic VS
    Biomaterials, 2009;30(36):6940-6. 2009
  10. Lysophosphatidic acid-induced nuclear localization of protein kinase C delta in bovine theca cells stimulated with luteinizing hormone.
    Authors: Budnik LT, Mukhopadhyay AK
    Biol. Reprod., 2002;67(3):935-44. 2002

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