DEPTOR/DEPDC6 Antibody

Novus Biologicals | Catalog # NBP2-27083

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Equine, Primate

Predicted:

Bovine (93%), Mouse (93%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DEPTOR/DEPDC6 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 230-300 of human DEPOR/DEPDC6 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 87% homologous in chicken.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DEPTOR/DEPDC6 Antibody

Western Blot: DEPTOR/DEPDC6 Antibody [NBP2-27083]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP2-27083]

Western Blot: DEPTOR/DEPDC6 Antibody [NBP2-27083] - Analysis of DEPOR/DEPDC6 in human lung lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27083 at 3 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for DEPTOR/DEPDC6 Antibody

Application
Recommended Usage

Western Blot

3 - 5 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DEPTOR/DEPDC6

DEP domain-containing mTOR-interacting protein (DEPOR/DEPTOR/DEPDC6) is a negative regulator of the mTORC1 and mTORC2 signaling pathways that regulate cell growth, proliferation and survival. It has two DEP domains and a PDZ (DHR) domain. Loss of DEPTOR activates S6k1, Akt, and SGk1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR overexpression suppresses S6k1 but, by relieving feedback inhibition from mTORC1 to PI3k signaling, activates Akt. Consistent with many human cancers having activated mTORC1 and mTORC2 pathways, DEPTOR expression is low in most cancers. Surprisingly, DEPTOR is highly overexpressed in a subset of multiple myelomas harboring cyclin D1/D3 or c-MAF/MAFB translocations. In these cells, high DEPTOR expression is necessary to maintain PI3k and Akt activation and a reduction in DEPTOR levels leads to apoptosis. It has also been reported that DEPOR has some functional significance in Lipid Biosynthesis.

Long Name

DEP Domain Containing mTOR-interacting Protein

Alternate Names

DEP.6, DEPDC6

Gene Symbol

DEPTOR

UniProt

Q8TB45 (Human)

Additional DEPTOR/DEPDC6 Products

Product Documents for DEPTOR/DEPDC6 Antibody

Certificate of Analysis

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Product Specific Notices for DEPTOR/DEPDC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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