Desmin Antibody (MSVA-651M)

Novus Biologicals | Catalog # NBP3-57230

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-651M
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Product Specifications

Immunogen

Recombinant full-length human desmin protein Swissprot P17661

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Appendix: Smooth muscle cells in the lamina muscularis mucosae and muscularis propria must show a strong cytoplasmic staining while staining should be at least moderate in most smooth muscle cells of blood vessels and in dispersed myofibroblasts..

Negative Control
Negitve control:Appendix: Desmin staining should not be seen in lymphocytes and epithelial cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Desmin Antibody (MSVA-651M)

Desmin Antibody (MSVA-651M)

Immunohistochemistry-Paraffin:Desmin Antibody (MSVA-651M)NBP3-57230

Rectum Strong desmin staining of lamina muscularis mucosae.
Desmin Antibody (MSVA-651M)

Immunohistochemistry-Paraffin:Desmin Antibody (MSVA-651M)NBP3-57230

Soft tissue Strong desmin positivity of all tumor cells of a rhabdomyosarcoma.
Desmin Antibody (MSVA-651M)

Immunohistochemistry-Paraffin:Desmin Antibody (MSVA-651M)NBP3-57230

Stomach Gastric adenocarcinoma invading desmin positive smooth muscle fibres.
Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Cancer Tissue Gallery

Desmin Cancer Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Cancer Tissue Gallery

Desmin Cancer Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Normal Tissue Gallery

Desmin Normal Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Normal Tissue Gallery

Desmin Normal Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Normal Tissue Gallery

Desmin Normal Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Normal Tissue Gallery

Desmin Normal Tissue Gallery

Desmin Antibody (MSVA-651M) Desmin Antibody (MSVA-651M) Desmin Normal Tissue Gallery

Desmin Normal Tissue Gallery

Applications for Desmin Antibody (MSVA-651M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Desmin

Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle.DE-U-10 labels desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas) (Debus E et al.).

Alternate Names

CMD1I, DES

Gene Symbol

DES

Additional Desmin Products

Product Documents for Desmin Antibody (MSVA-651M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Desmin Antibody (MSVA-651M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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