Desmoglein-2 Antibody

Novus Biologicals | Catalog # NBP1-33374

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Desmoglein-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Desmoglein-2. The exact sequence is proprietary.

Reactivity Notes

Bovine (87%).

Localization

Cell membrane, Cell junction, desmosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Desmoglein-2 Antibody (NBP1-33374) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Desmoglein-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Desmoglein-2 Antibody

Western Blot: Desmoglein-2 Antibody [NBP1-33374]

Western Blot: Desmoglein-2 Antibody [NBP1-33374]

Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Desmoglein-2 Antibody [NBP1-33374]

Immunocytochemistry/ Immunofluorescence: Desmoglein-2 Antibody [NBP1-33374]

Immunocytochemistry/Immunofluorescence: Desmoglein-2 Antibody [NBP1-33374] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Human colon cancer. Desmoglein 2 stained by Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Desmoglein-2 Antibody [NBP1-33374]

Western Blot: Desmoglein-2 Antibody [NBP1-33374]

Western Blot: Desmoglein-2 Antibody [NBP1-33374] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Desmoglein-2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - DU145 xenograft, using Desmoglein-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374]

Immunohistochemistry-Paraffin: Desmoglein-2 Antibody [NBP1-33374] - Mouse skin. Desmoglein 2 antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Desmoglein-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Desmoglein-2

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.

Alternate Names

CDHF5, Desmoglein2, DSG2, HDGC

Gene Symbol

DSG2

Additional Desmoglein-2 Products

Product Documents for Desmoglein-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Desmoglein-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Desmoglein-2 Antibody

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Protocols

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