Desmoglein-3 Antibody (6G2C11) - BSA Free

Novus Biologicals | Catalog # NBP2-37259

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6G2C11

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human Desmoglein-3 (AA: 55-159) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

107.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Desmoglein-3 Antibody (6G2C11) - BSA Free

Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mouse mAb against A431 cell lysate.
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Analysis of esophageal tissues using DSG3 mouse mAb with DAB staining.
Flow Cytometry: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Flow Cytometry: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Flow Cytometry: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Flow cytometric analysis of A431 cells using DSG3 mouse mAb (green) and negative control (red).
Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mAb against human DSG3 (AA: 55-159) recombinant protein. (Expected MW is 37.5 kDa)
Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11)BSA Free [NBP2-37259]

Western Blot: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Western blot analysis using DSG3 mAb against HEK293 (1) and DSG3 (AA: 55-159)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Analysis of esophageal cancer tissues using DSG3 mouse mAb with DAB staining.
ELISA: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

ELISA: Desmoglein-3 Antibody (6G2C11) - BSA Free [NBP2-37259]

ELISA: Desmoglein-3 Antibody (6G2C11) [NBP2-37259] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Desmoglein-3 Antibody (6G2C11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Desmoglein-3

Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris.

Alternate Names

CDHF6, Desmoglein3, DSG3

Gene Symbol

DSG3

Additional Desmoglein-3 Products

Product Documents for Desmoglein-3 Antibody (6G2C11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Desmoglein-3 Antibody (6G2C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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