DFF40/CAD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76948

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DFF40/CAD Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40. Amino Acid Squence: GLESRFRNKSGYLRYSC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DFF40/CAD Antibody - BSA Free

Western Blot: DFF40/CAD AntibodyBSA Free [NBP1-76948]

Western Blot: DFF40/CAD AntibodyBSA Free [NBP1-76948]

Western Blot: DFF40/CAD Antibody [NBP1-76948] - Analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF40/CAD (I18) using NBP1-76948 at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948]

Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948]

Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Jurkat cells with DFF40 antibody at 5 ug/ml.
DFF40/CAD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] -

Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-76948] - Immunofluorescence of DFF40/CAD in K562 cells with DFF40/CAD antibody at 20 ug/mL.

Green: DFF40/CAD Antibody
Blue: DAPI staining

Applications for DFF40/CAD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Western Blot

0.5 ug/mL
Application Notes
A 40 kDa band can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DFF40/CAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Long Name

DNA Fragmentation Factor, Subunit beta

Alternate Names

CAD, CPAN, DFF2, DFFB

Gene Symbol

DFFB

UniProt

NP_031885 (Mouse)

Additional DFF40/CAD Products

Product Documents for DFF40/CAD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DFF40/CAD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DFF40/CAD Antibody - BSA Free

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  • Q: Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?

    A: CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

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