DFF45/ICAD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77034

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DFF45/ICAD Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45. Amino Acid Squence: KASPPGDLQNPKRARQDPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DFF45/ICAD Antibody - BSA Free

Western Blot: DFF45/ICAD AntibodyBSA Free [NBP1-77034]

Western Blot: DFF45/ICAD AntibodyBSA Free [NBP1-77034]

Western Blot: DFF45/ICAD Antibody [NBP1-77034] - Analysis of HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with ICAD antibody at 1:1000 dilution.
DFF45/ICAD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP1-77034] -

Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP1-77034] - Immunocytochemistry of DFF45/ICAD in HeLa cells with DFF45/ICAD antibody at 5 u/mL.
DFF45/ICAD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP1-77034] -

Immunocytochemistry/ Immunofluorescence: DFF45/ICAD Antibody - BSA Free [NBP1-77034] - Immunofluorescence of DFF45/ICAD in Hela cells with DFF45/ICAD antibody at 20 u/mL.

Green: DFF45/ICAD Antibody
Blue: DAPI staining

Applications for DFF45/ICAD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Western Blot

0.5-1 ug/ml
Application Notes
A 45 kDa band can be detected in non-apoptotic cells in Western Blot.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DFF45/ICAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

Long Name

DNA Fragmentation Factor, Subunit alpha

Alternate Names

DFF1, DFFA, ICAD

Gene Symbol

DFFA

UniProt

NP_004392 (Human)

Additional DFF45/ICAD Products

Product Documents for DFF45/ICAD Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DFF45/ICAD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Adaptor Proteins

Citations for DFF45/ICAD Antibody - BSA Free

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Protocols

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