Dfna5 Antibody

Western Blot: Dfna5 Antibody [NBP2-80426]

Western Blot: Dfna5 Antibody [NBP2-80426] - Analysis of Dfna5 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80426, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426]

Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in mouse small intestine tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.

Flow Cytometry: Dfna5 Antibody [NBP2-80426]

Flow Cytometry: Dfna5 Antibody [NBP2-80426] - Analysis of Methionine Aminopeptidase 2/METAP2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).