DGK-epsilon Antibody (7E1) - Azide and BSA Free

Novus Biologicals | Catalog # H00008526-M03

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Lambda Clone # 7E1

Format

Azide and BSA Free
View all DGK-epsilon Primary Antibodies »

Product Specifications

Immunogen

DGKE (NP_003638, 141 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEF

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

DGKE - diacylglycerol kinase, epsilon 64kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DGK-epsilon Antibody (7E1) - Azide and BSA Free

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in Raw 264.7 (Cat # L024V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in HeLa (Cat # L013V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in PC-12 (Cat # L012V1).
Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03]

Western Blot: DGK-epsilon Antibody (7E1) [H00008526-M03] - Analysis of DGKE expression in NIH/3T3 (Cat # L018V1).
Sandwich ELISA: DGK-epsilon Antibody (7E1) [H00008526-M03] - Detection limit for recombinant GST tagged DGKE is 0.3 ng/ml as a capture antibody.

Applications for DGK-epsilon Antibody (7E1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23542698).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DGK-epsilon

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.

Long Name

Diacylglycerol Kinase, epsilon 64kDa

Alternate Names

DAGK5, DAGK6, DGKE, DGKepsilon

Entrez Gene IDs

8526 (Human)

Gene Symbol

DGKE

OMIM

601440 (Human)

UniProt

NP_003638 (Human)

Additional DGK-epsilon Products

Product Documents for DGK-epsilon Antibody (7E1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DGK-epsilon Antibody (7E1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for DGK-epsilon Antibody (7E1) - Azide and BSA Free

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Protocols

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FAQs

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