DHFRL1 Antibody (OTI1E2)

Novus Biologicals | Catalog # NBP2-01019

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Canine, Monkey

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E2
View all DHFRL1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 4-185 of human DHFRL1(NP_789785) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DHFRL1 Antibody (OTI1E2)

Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DHFRL1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Immunocytochemistry/ Immunofluorescence: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Immunocytochemistry/Immunofluorescence: DHFRL1 Antibody (OTI1E2) [NBP2-01019] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFRL1.
Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019]

Western Blot: DHFRL1 Antibody (OTI1E2) [NBP2-01019] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFRL1.

Applications for DHFRL1 Antibody (OTI1E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DHFRL1

Alternate Names

DHFRP4, dihydrofolate reductase pseudogene 4, dihydrofolate reductase-like 1, dihydrofolate reductase-like protein 1, EC 1.5.1.-, EC 1.5.1.3, FLJ16119

Entrez Gene IDs

200895 (Human)

Gene Symbol

DHFR2

Additional DHFRL1 Products

Product Documents for DHFRL1 Antibody (OTI1E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DHFRL1 Antibody (OTI1E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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