DHX58 Antibody

Novus Biologicals | Catalog # NBP1-20939

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DHX58 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence DFLQHCAENLSD corresponding to C-Terminus according to NP_077024.2.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DHX58 Antibody

Western Blot: DHX58 Antibody [NBP1-20939]

Western Blot: DHX58 Antibody [NBP1-20939]

Western Blot: DHX58 Antibody [NBP1-20939] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939]

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939]

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939]

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939]

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939] - Staining of paraffin embedded Human Cortex. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for DHX58 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 80 kDa band observed in human liver lysates (calculated MW of 76.6 kDa band according to NP_077024.2). An additional band of unknown identity was also consistently observed at 24 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DHX58

Negative regulator of host innate immune defense against viruses. The repressor domain of DHX58 interactswith DDX58 and negatively regulates DDX58-mediated signaling. Binds dsRNA produced during viral replication, inparticuliar HCV RNA. Binding occurs at blunt dsRNA termini

Alternate Names

D11LGP2E, D11LGP2probable ATP-dependent RNA helicase DHX58, DEXH (Asp-Glu-X-His) box polypeptide 58, EC 3.6.4.13, LGP2ortholog of mouse D11lgp2, Probable ATP-dependent helicase LGP2, Protein D11Lgp2 homolog, RIG-I-like receptor LGP2, RLR, RNA helicase LGP2

Entrez Gene IDs

79132 (Human)

Gene Symbol

DHX58

UniProt

NP_077024.2 (Human)

Additional DHX58 Products

Product Documents for DHX58 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DHX58 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DHX58 Antibody

There are currently no reviews for this product. Be the first to review DHX58 Antibody and earn rewards!

Have you used DHX58 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies