Dihydrofolate Reductase/DHFR Antibody (9B2) - BSA Free

Novus Biologicals | Catalog # NBP3-15195

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9B2 expressed in HEK293

Format

BSA Free
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Dihydrofolate Reductase/DHFR. UniProt ID (P00374).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (9B2) - BSA Free

Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: Dihydrofolate Reductase/DHFR antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 16 KDa. Observed band size: 22 KDa.
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Immunoprecipitating Dihydrofolate Reductase/DHFR in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of Dihydrofolate Reductase/DHFR antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: Dihydrofolate Reductase/DHFR antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

Applications for Dihydrofolate Reductase/DHFR Antibody (9B2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (9B2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (9B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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