Dihydrofolate Reductase/DHFR Antibody (SR1325) - BSA Free

Novus Biologicals | Catalog # NBP3-21772

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1325 expressed in HEK293

Format

BSA Free
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Dihydrofolate Reductase/DHFR (Uniprot #: P00374)

Specificity

Detects endogenous levels of total Dihydrofolate Reductase/DHFR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody (SR1325) - BSA Free

Western Blot: Dihydrofolate Reductase/DHFR Antibody (SR1325) [NBP3-21772] -

Western Blot: Dihydrofolate Reductase/DHFR Antibody (SR1325) [NBP3-21772] - Western blot analysis using NBP3-21772 in (1) HeLa cell lysate; (2) Mouse spleen lysate.

Applications for Dihydrofolate Reductase/DHFR Antibody (SR1325) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody (SR1325) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody (SR1325) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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FAQs

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