Dihydrofolate Reductase/DHFR Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16163

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dihydrofolate Reductase/DHFR Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dihydrofolate Reductase/DHFR. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Bovine (85%), Porcine (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dihydrofolate Reductase/DHFR Antibody - BSA Free

Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGE DHFR antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunocytochemistry/ Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunocytochemistry/Immunofluorescence: Dihydrofolate reductase Antibody [NBP2-16163] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunohistochemistry-Paraffin: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunohistochemistry-Paraffin: Dihydrofolate reductase Antibody [NBP2-16163] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163]

Immunoprecipitation: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 ug of DHFR antibody. Western blot analysis was performed using DHFR antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Dihydrofolate Reductase/DHFR Antibody

Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] -

Western Blot: Dihydrofolate Reductase/DHFR Antibody [NBP2-16163] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Dihydrofolate Reductase/DHFR antibody (NBP2-16163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Dihydrofolate Reductase/DHFR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dihydrofolate Reductase/DHFR

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq]

Alternate Names

DHFR, DHFRP1, DYR

Gene Symbol

DHFR

UniProt

P00374 (Human)

Additional Dihydrofolate Reductase/DHFR Products

Product Documents for Dihydrofolate Reductase/DHFR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Dihydrofolate Reductase/DHFR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

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