Dihydrolipoamide Dehydrogenase/DLD Antibody

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] -

Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody [N1N3] (NBP2-19361) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] -

Sample preparation and differentially abundant proteins in DM and NDM tissues. Sample preparation was reproducible and similar between groups, as determined by the number of proteins quantified (a) and principal component analysis (b); no significant difference between groups was observed in number of proteins quantified; principal component projection does not separate DM from NDM samples indicating that overall protein composition of samples is similar between groups. (c) Volcano plots of protein abundance in DM compared with NDM samples. Light grey dots indicate differentially abundant proteins between groups; black dots represent proteins that were not significantly regulated. Labels indicate gene symbols. (d) Western blots for select targets differentially regulated in proteomic analysis. (e) Western blotting densitometry quantification. *p-value < 0.001, independent t-test comparing NDM (n = 8) to DM (n = 8) densitometry values, normalized to actin as described in methods. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34462518), licensed under a CC-BY license. Not internally tested by Novus Biologicals.