Directed In Vivo Angiogenesis Assay Starter Kit

Catalog # Availability Size / Price Qty
3450-048-SK
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Directed In Vivo Angiogenesis Assay Starter Kit Summary

The DIVAA Starter Kit contains 48 Angioreactors and enough growth factor to induce angiogenesis in all 48 angio-reactors.

Why Use Directed In Vivo Angiogenesis Assay Starter Kit?

DIVAA is the first in vivo system for the study of angiogenesis that provides quantitative and reproducible results. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with 20 μl of Cultrex Basement Membrane Extract (BME) premixed with or without angiogenic-modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flank of nude mice. Accompanied with the onset of angiogenesis, vascular endothelial cells proceed to grow into the BME and form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic modulating factors. The sleek design of the angioreactor provides a standardized platform for reproducible and quantifiable in vivo angiogenesis assays. Compared to the plug assay, the angioreactor prevents assay errors due to absorption of the basement membrane extract by the mouse. In addition, the angioreactor uses only a fraction of the materials conserving both BME and test compounds used, and up to four angioreactors may be implanted in each mouse, allowing for greater statistical power. DIVAA has been used in a variety of investigations.

Kit Contents

• DIVAA BME
• DIVAA FGF-2 Positive Control
• DIVAA Cell Sperse
• DIVAA FGF-2 (1800ng)/ VEGF (600ng)
• DIVAA Angioreactor
• DIVAA AngioRack (must be autoclaved prior to use)
• DIVAA Wash Buffer
• DIVAA FITC-Lectin 200X
• 25X FITC-Lectin Diluent
• Heparin Solution

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

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