Directed In Vivo Angiogenesis Assay Starter Kit

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Directed In Vivo Angiogenesis Assay Starter Kit Summary

The Cultrex® Directed In Vivo Angiogenesis Assay (DIVAA™) is designed to assess angiogenesis in an in vivo system. The DIVAA Starter Kit is designed to give new users practical experience using this assay system and is ideal for optimizing assay conditions. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with basement membrane extract (BME) premixed with or without angiogenesis modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flanks of nude mice. If filled with angiogenic factors, vascular endothelial cells migrate into, and proliferate in the BME to form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic factors. Each kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all of the provided angioreactors. The DIVAA Starter Kit also supplies an autoclavable and reusable AngioRack™ to hold the angioreactors while they are being prepared for implantation.


  • Utilizes a standardized platform for reproducible and quantifiable results
  • Results seen in as few as 9 days post-implantation
  • Up to four angioreactors can be implanted in each mouse, allowing for greater statistical power
  • Uses a fraction of materials conserving BME and test compounds
  • Averts assay errors caused by absorption of BME by the mouse

Kit Contents

  • Angioreactors
  • Growth Factor Reduced PathClear® BME
  • 10X Wash Buffer
  • FGF-2
  • CellSperse™
  • 200X FITC-Lectin
  • 25X FITC-Lectin Diluent
  • Heparin Solution
  • FGF-2 (1.8 μg)/VEGF (600 ng)

Evaluation of Angiogenesis Activation using DIVAA. DIVAA was used to assess angiogenesis in nude mice stimulated by either fibroblast growth factor-2 alone (FGF-2, 100 ng) or in combination with vascular endothelial growth factor (FGF-2, 37 ng; VEGF, 12.5 ng). Angiogenesis was measured as the amount of FITC-Lectin detected 9 days post-implantation. Data is shown as the amount of fluorescence detected in treated samples (FGF-2, FGF-2/VEGF) versus the negative control (Untreated).

Cultrex and PathClear are registered trademarks of Trevigen, Inc.
DIVAA, AngioRack, and CellSperse are trademarks of Trevigen, Inc.

Product Datasheets

Preparation and Storage

Stability & Storage
The product  requires storage at both -20 °C and 2 to 8 °C.  Consult the product insert for specific storage temperatures. Do not use past expiration date.

Background: Angiogenesis Assay Kits

Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.

Alternate Names
Angiogenesis Assay Kits

Citations for Directed In Vivo Angiogenesis Assay Starter Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Correlation of two distinct metastasis-associated proteins, MTA1 and S100A4, in angiogenesis for promoting tumor growth
    Authors: M Ishikawa, M Osaki, M Yamagishi, K Onuma, H Ito, F Okada, H Endo
    Oncogene, 2019;0(0):.  2019
  2. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
    Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
    J Biol Chem, 2015;290(36):22212-24.  2015
  3. beta-hairpin peptide that targets vascular endothelial growth factor (VEGF) receptors: design, NMR characterization, and biological activity.
    Authors: Diana D, Basile A, De Rosa L, Di Stasi R, Auriemma S, Arra C, Pedone C, Turco M, Fattorusso R, D'Andrea L
    J Biol Chem, 0;286(48):41680-91.  0
  4. High Aldehyde Dehydrogenase Activity Identifies a Subset of Human Mesenchymal Stromal Cells with Vascular Regenerative Potential.
    Authors: Sherman S, Kuljanin M, Cooper T, Putman D, Lajoie G, Hess D
    Stem Cells, 0;35(6):1542-1553.  0
  5. Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
    Authors: Vuong T, Reine T, Sudworth A, Jenssen T, Kolset S
    J Histochem Cytochem, 0;63(4):280-92.  0
  6. Azithromycin effectively inhibits tumor angiogenesis by suppressing vascular endothelial growth factor receptor 2-mediated signaling pathways in lung cancer.
    Authors: Li F, Huang J, Ji D, Meng Q, Wang C, Chen S, Wang X, Zhu Z, Jiang C, Shi Y, Liu S, Li C
    Oncol Lett, 0;14(1):89-96.  0
  7. Embryonic protein nodal promotes breast cancer vascularization.
    Authors: Quail D, Walsh L, Zhang G, Findlay S, Moreno J, Fung L, Ablack A, Lewis J, Done S, Hess D, Postovit L
    Cancer Res, 0;72(15):3851-63.  0
  8. Sphingosine-1-phosphate produced by sphingosine kinase 1 promotes breast cancer progression by stimulating angiogenesis and lymphangiogenesis.
    Authors: Nagahashi M, Ramachandran S, Kim E, Allegood J, Rashid O, Yamada A, Zhao R, Milstien S, Zhou H, Spiegel S, Takabe K
    Cancer Res, 0;72(3):726-35.  0


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