DIVAA Angioreactor with Cultrex RGF BME
DIVAA Angioreactor with Cultrex RGF BME SummaryDIVAA Angioreactors and Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) are used together as an implantable devices that provides a standardized platform to generate reproducible and quantifiable angiogenesis in vivo.
Why Use DIVAA Angioreactor with Cultrex RGF BME?
DIVAA Angioreactors are one centimeter long cylinders that is sealed on one end and houses 20 μl total volume. They are made of implant-grade silicone and provided sterile. Angioreactors are filled with Cultrex Basement Membrane Extract (BME)* premixed with or without angiogenic-modulating factors and implanted subcutaneously in the dorsal flank of nude mice. Through the process of angiogenesis, host vascular endothelial cells proceed to invade and grow into the BME, forming vessels in the angioreactor. The sleek design of the angioreactor provides a standardized platform for reproducible and quantifiable in vivo angiogenesis assays. Compared to the plug assay, the angioreactor prevents assay errors due to absorption of the basement membrane extract by the mouse. In addition, the angioreactor uses only a fraction of the materials conserving both BME and test compounds used, and up to four angioreactors may be implanted in each mouse, allowing for greater statistical power. These are an essential component of the Directed In Vivo Angiogenesis Assays (DIVAA).
• DIVAA Cultrex BME
• DIVAA Angioreactor
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
For research use only. Not for diagnostic use.
Around the angioreactor in the mouse, a capsule forms with time. When harvesting the angioreactor, it is difficult to dissect the capsule tissue from the opening of the angioreactor. There is a plug of tissue growing into the chamber. Is this normal? Do you have any recommendations on how to handle chambers like this?
Growth of connective tissue into the angioreactor during angiogenesis is normal. The amount of connective tissue is dependent on the strain of mice, treatment, and response. The cell dissociation step may be increased up to 3 hours to improve dissociation/degradation, and any remaining connective tissue may be removed. If this does not solve the problem, it may be more appropriate to use the Optional Protocol for Dextran-FITC Detection (which measures capillary volume and discards cellular/connective tissue debris).
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