Q: Around the angioreactor in the mouse, a capsule forms with time. When harvesting the angioreactor, it is difficult to dissect the capsule tissue from the opening of the angioreactor. There is a plug of tissue growing into the chamber. Is this normal? Do you have any recommendations on how to handle chambers like this?

A: Growth of connective tissue into the angioreactor during angiogenesis is normal. The amount of connective tissue is dependent on the strain of mice, treatment, and response. The cell dissociation step may be increased up to 3 hours to improve dissociation/degradation, and any remaining connective tissue may be removed. If this does not solve the problem, it may be more appropriate to use the Optional Protocol for Dextran-FITC Detection (which measures capillary volume and discards cellular/connective tissue debris).

DIVAA Angioreactor with Cultrex RGF BME

R&D Systems | Catalog # 3450-048-DA

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Discontinued Product

3450-048-DA has been discontinued. View all Angiogenesis Assay Kits products.

Product Summary
Kit Contents
Preparation & Storage
Background
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Key Product Details

Features

DIVAA Angioreactors and Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) are used together as an implantable devices that provides a standardized platform to generate reproducible and quantifiable angiogenesis in vivo.

Product Summary for DIVAA Angioreactor with Cultrex RGF BME

Why Use DIVAA Angioreactor with Cultrex RGF BME?

DIVAA Angioreactors are one centimeter long cylinders that is sealed on one end and houses 20 μl total volume. They are made of implant-grade silicone and provided sterile. Angioreactors are filled with Cultrex Basement Membrane Extract (BME)* premixed with or without angiogenic-modulating factors and implanted subcutaneously in the dorsal flank of nude mice. Through the process of angiogenesis, host vascular endothelial cells proceed to invade and grow into the BME, forming vessels in the angioreactor. The sleek design of the angioreactor provides a standardized platform for reproducible and quantifiable in vivo angiogenesis assays. Compared to the plug assay, the angioreactor prevents assay errors due to absorption of the basement membrane extract by the mouse. In addition, the angioreactor uses only a fraction of the materials conserving both BME and test compounds used, and up to four angioreactors may be implanted in each mouse, allowing for greater statistical power. These are an essential component of the Directed In Vivo Angiogenesis Assays (DIVAA).

Kit Contents for DIVAA Angioreactor with Cultrex RGF BME

DIVAA Cultrex BME
DIVAA Angioreactor

Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Angiogenesis Assay Kits

Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.

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