DIVAA FGF-2 VEGF
DIVAA FGF-2 VEGF Summary
Why Use DIVAA FGF-2 (1.8 µg)/VEGF(600 ng)?
DIVAA FGF-2 (1800ng)/ VEGF (600ng) is provided in 60 µl at a concentration of 30 µg/mL FGF-2 and 10 µg/mL VEGF. This solution is diluted 20-fold when suspended in Cultrex RGF BME for a final concentration of 1.5 µg/mL FGF-2 and 500 ng/mL VEGF. Recombinant Mouse VEGF 164 is expressed by baculovirus, and recombinant FGF-2 is expressed in E. coli. FGF-2 / VEGF provides a stronger angiogenic response compared to the FGF-2 Positive Control for the Directed In Vivo Angiogenesis Assay (DIVAA).
For research use only. Not for diagnositic use.
Around the angioreactor in the mouse, a capsule forms with time. When harvesting the angioreactor, it is difficult to dissect the capsule tissue from the opening of the angioreactor. There is a plug of tissue growing into the chamber. Is this normal? Do you have any recommendations on how to handle chambers like this?
Growth of connective tissue into the angioreactor during angiogenesis is normal. The amount of connective tissue is dependent on the strain of mice, treatment, and response. The cell dissociation step may be increased up to 3 hours to improve dissociation/degradation, and any remaining connective tissue may be removed. If this does not solve the problem, it may be more appropriate to use the Optional Protocol for Dextran-FITC Detection (which measures capillary volume and discards cellular/connective tissue debris).
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