Dkk-3 Antibody (1R5E0)

Novus Biologicals | Catalog # NBP3-16293

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1R5E0 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-350 of human Dkk-3 (NP_056965.3). TRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dkk-3 Antibody (1R5E0)

Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293]

Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293]

Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293] - Western blot analysis of extracts of various cell lines, using Dkk-3 Rabbit mAb (NBP3-16293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293]

Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293]

Immunocytochemistry/Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293] - Immunofluorescence analysis of SH-SY5Y cells using Dkk-3 Rabbit mAb (NBP3-16293) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Dkk-3 Antibody (1R5E0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dkk-3

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Gene Symbol

DKK3

Additional Dkk-3 Products

Product Documents for Dkk-3 Antibody (1R5E0)

Certificate of Analysis

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Product Specific Notices for Dkk-3 Antibody (1R5E0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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