DLX5 Antibody

Novus Biologicals | Catalog # NBP1-28911

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DLX5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AYNRVPSATNQPEK corresponding to internal region according to NP_005212.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DLX5 Antibody

Western Blot: DLX5 Antibody [NBP1-28911]

Western Blot: DLX5 Antibody [NBP1-28911]

Western Blot: Dlx5 Antibody [NBP1-28911] - (1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DLX5 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
Western blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5;278(36):34387-94; PMID: 12815054).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DLX5

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq]

Long Name

Distal-less Homeobox 5

Alternate Names

distal-less homeo box 5, distal-less homeobox 5, homeobox protein DLX-5

Entrez Gene IDs

1749 (Human); 13395 (Mouse); 25431 (Rat)

Gene Symbol

DLX5

UniProt

NP_005212.1 (Human)

Additional DLX5 Products

Product Documents for DLX5 Antibody

Certificate of Analysis

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Product Specific Notices for DLX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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