Q: Why is HEPES used in cell culture?

A: HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid, pKa@37oC=7.3, useful range pH 6.6 – 8.0) is one of several biological buffers that can be used to stabilize the pH of cell culture medium. As cells grow and metabolize the cell culture medium nutrients, they typically produce excess acid – that lowers the pH of the medium. This lowering of pH can be detrimental to the continued growth of cells in culture. HEPES provides more “buffering” capacity than the bicarbonate normally found in cell culture medium – thus slowing the decline in pH as cells metabolize the nutrient medium. For some cell lines this extends the time between “feedings” and promotes healthier cell growth. HEPES can show toxicity with some cell lines, so it is not a universal panacea. Other useful biological buffers include MOPS (pKa@37oC=7.0), TRIS (pKa@37oC=7.8), and PIPES (pKa@37oC=6.7).

DMEM/F-12, 15 mM HEPES, no glutamine, no phenol red

R&D Systems | Catalog # M23550

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Key Product Details

Features

Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) with 15 mM HEPES buffer. Without L-glutamine and phenol red.

Applications

Cell Culture

Product Summary for DMEM/F-12, 15 mM HEPES, no glutamine, no phenol red

What is DMEM/F-12 Media?

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F-12 (DMEM/F-12) is a 1:1 mixture of DMEM and F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham’s Nutrient Mixture F-12’s additional components, was found to support the growth of a wide range of mammalian cells. DMEM/F-12 requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.

Each lot of DMEM/F-12 is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of DMEM/F-12 are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. DMEM/F-12 is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the datasheet.

Product Specifications

Sterility Testing

Pass

Formulation, Preparation, and Storage

Purification

0.2 μm filtered

Shipping

Ambient

Storage

2 - 8 °C, protected from light

Product Documents for DMEM/F-12, 15 mM HEPES, no glutamine, no phenol red

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