DMEM, high glucose, GlutaminePlus, sodium pyruvate
DMEM, high glucose, GlutaminePlus, sodium pyruvate Summary
What is DMEM?
Dulbecco’s Modified Eagle’s Medium (DMEM), a modification of Eagle’s BME, is widely used for a range of mammalian cell culture applications. In comparison with BME, it contains a four-fold higher concentration of amino acids, vitamins, and additional components. A variety of DMEM versions are available in high glucose and low glucose formulations. DMEM requires supplementation with serum, typically 10% Fetal Bovine Serum (FBS), since this medium does not contain proteins, lipids, or growth factors.
Each lot of DMEM is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of DMEM are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. DMEM is manufactured in our ISO 9001:2015 certified facility.
For the specific media formulation, please refer to the datasheet.
For research use only. Not for diagnositic use.
What is GlutaminePlus, and how does it compare to L-Glutamine?
GlutaminePlus, a derivative of L-glutamine obtained by a chemical reaction of L-alanine with L-glutamine, is available in liquid form or as an ingredient in ready-to-use Atlanta Biologicals brand cell culture media (media with stable L-glutamine).
GlutaminePlus is metabolized within the cells to yield L-glutamine plus the second amino acid. This results in more consistent delivery of L-glutamine to cells in culture and avoids toxic buildup of ammonia in cell cultures. This feature can be especially important for ammonia sensitive cell lines.
L-glutamine is an essential amino acid and plays a major role for the growth and function of cells in culture. Although L-glutamine is stable in crystalline form, it has the tendency to degrade non-enzymatically and irreversibly in solution within a short time period. The rate of this L-glutamine breakdown is dependent on pH, temperature and the presence of various anions.
One of the by-products, ammonia, may act as a toxin or growth inhibitor for the cultured cells. Because of its chemical instability and significance for cell growth, it is important that the delivery of L-glutamine be optimized to each specific cell culture application. A recommended way of achieving reliable delivery of L-glutamine to the cells in culture is the use of stable derivatives of L-glutamine in cell culture media.
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