DMTF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84073

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DMTF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASPTVTLTAAAPASPEQIIVHALSPEHLLNTSDNVTVQCHTPRVIIQTVATEDITSSISQAELTVDSDIQSSDFPEPPDALEADTFPDEIHHPKMTVEPSFNDAHVSKFSDQNSTELMNSVMVRTEEEI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DMTF1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DMTF1 Antibody [NBP1-84073]

Immunocytochemistry/ Immunofluorescence: DMTF1 Antibody [NBP1-84073]

Immunocytochemistry/Immunofluorescence: DMTF1 Antibody [NBP1-84073] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073]

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073]

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
DMTF1 Antibody

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073]-

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073]- Staining of human small intestine shows strong nuclear positivity in glandular cells.
DMTF1 Antibody

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073] -

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073] -Staining of human endometrium shows strong nuclear positivity in glandular cells.
DMTF1 Antibody

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073] -

Immunohistochemistry-Paraffin: DMTF1 Antibody [NBP1-84073] -Staining of human cerebral cortex shows strong nuclear positivity in neurons.
DMTF1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: DMTF1 Antibody - BSA Free [NBP1-84073]

Chromatin Immunoprecipitation-exo-Seq: DMTF1 Antibody - BSA Free [NBP1-84073]

ChIP-Exo-Seq composite graph for Anti-DMTF1 (NBP1-84073) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for DMTF1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DMTF1

DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

cyclin D binding myb-like transcription factor 1, cyclin D-binding Myb-like protein 1, cyclin-D-binding Myb-like transcription factor 1, Cyclin-D-interacting Myb-like protein 1, DMP1FLJ76054, FLJ25188, FLJ41265, hDMP1DMTF, hDMTF1

Gene Symbol

DMTF1

Additional DMTF1 Products

Product Documents for DMTF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DMTF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DMTF1 Antibody - BSA Free

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Protocols

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