DNA Ligase III Antibody (1F3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41190

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human

Applications

Validated:

Western Blot, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F3

Format

Azide and BSA Free
View all DNA Ligase III Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human DNA Ligase III-b.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear and Mitochondrial

Specificity

This antibody will recognize human DNA ligase III.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Ligase III Antibody (1F3) - Azide and BSA Free

Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]

Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]

Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]

Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]

Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. DNA ligase III dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Applications for DNA Ligase III Antibody (1F3) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunoprecipitation

Assay dependent

Proximity Ligation Assay

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Ligase III

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (beta-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

Long Name

DNA ligase 3

Alternate Names

LIG3

Entrez Gene IDs

3980 (Human)

Gene Symbol

LIG3

Additional DNA Ligase III Products

Product Documents for DNA Ligase III Antibody (1F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DNA Ligase III Antibody (1F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA Ligase III Antibody (1F3) - Azide and BSA Free

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Protocols

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FAQs

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