DNA polymerase alpha Antibody

Name: DNA polymerase alpha Antibody
Brand: Novus Biologicals
SKU: NBP1-47256
Price: 459 USD
Availability: InStock
Rating: 5 (1 reviews)

Novus Biologicals | Catalog # NBP1-47256

(1)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 775 and 825 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA polymerase alpha Antibody

Western Blot: DNA polymerase alpha Antibody [NBP1-47256]

Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope

Immunocytochemistry/ Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256]

Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). Image courtesy of Dr. Matt Fuller at University of Missouri-Columbia.

Applications for DNA polymerase alpha Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from a verified customer review

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP1-47256 in the following applications:

Immunocytochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA polymerase alpha

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and episilon are involved in DNA replication and repair. DNA pol delta and DNA pol episilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

Long Name

DNA polymerase alpha catalytic subunit

Alternate Names

EC 2.7.7.7, p180, POLA, POLA1

Entrez Gene IDs

5422 (Human); 18968 (Mouse)

Gene Symbol

POLA1

UniProt

P09884 (Human)

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Product Specific Notices for DNA polymerase alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DNA polymerase alpha Antibody (1)

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Name: Anonymous

Application: Immunocytochemistry

Sample Tested: A9 fibroblasts

Species: Mouse

Verified Customer | Posted 07/11/2016

Mouse A9 fibroblasts stained with DNA polymerase alpha Antibody

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