DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-60438

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNA Polymerase epsilon subunit 3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 97 and 147 of human DNA polymerase epsilon subunit 3 using the numbering given in entry NP_059139.2 (GeneID 54107).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438]

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438]

Western Blot: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-60438 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438]

Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody [NB100-60438] - Section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PolE3/p17 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000- 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA Polymerase epsilon subunit 3

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]

Alternate Names

Arsenic-transactivated protein, AsTP, CHARAC17arsenic transactivated protein, CHRAC-17, CHRAC17Ybl1, Chromatin accessibility complex 17 kDa protein, DNA polymerase epsilon p17 subunit, DNA polymerase epsilon subunit 3, DNA polymerase epsilon subunit p17, DNA polymerase II subunit 3, EC 2.7.7.7, histone fold protein CHRAC17, HuCHRAC17, p17, polymerase (DNA directed), epsilon 3 (p17 subunit), YBL1

Gene Symbol

POLE3

UniProt

NP_059139.2 (Human)

Additional DNA Polymerase epsilon subunit 3 Products

Product Documents for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DNA Polymerase epsilon subunit 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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