DNCIC1 Antibody (74.1)

Novus Biologicals | Catalog # NB300-726

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Drosophila, Mammal, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 74.1
View all DNCIC1 Primary Antibodies »

Product Specifications

Immunogen

Purified bovine brain cytoplasmic dynein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

Detects two isoforms of the 74kDa polypeptide of cytoplasmic Dynein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for DNCIC1 Antibody (74.1)

Western Blot: DNCIC1 Antibody (74.1) [NB300-726]

Western Blot: DNCIC1 Antibody (74.1) [NB300-726]

Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of cytoplasmic dynein in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: DNCIC1 Antibody (74.1) [NB300-726]

Immunohistochemistry-Paraffin: DNCIC1 Antibody (74.1) [NB300-726]

Immunohistochemistry-Paraffin: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon tissue using anti-DNCIC1 antibody. Image from verified customer review.
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in C6 cells compared to an isotype control (blue).
Western Blot: DNCIC1 Antibody (74.1) [NB300-726]

Western Blot: DNCIC1 Antibody (74.1) [NB300-726]

Western Blot: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in human colon cancer cell lysates using anti-DNCIC1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - analysis of DNCIC1 in caco-2 cells using anti-DNCIC1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/ Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726]

Immunocytochemistry/Immunofluorescence: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dynein monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Dynein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in U87-MG cells compared to an isotype control (blue).
Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726]

Flow Cytometry: DNCIC1 Antibody (74.1) [NB300-726] - Analysis of Dynein in Hela cells compared to an isotype control (blue).

Applications for DNCIC1 Antibody (74.1)

Application
Recommended Usage

Flow Cytometry

1 - 2 ug

Immunocytochemistry/ Immunofluorescence

2 - 4 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml

Reviewed Applications

Read 3 reviews rated 4.7 using NB300-726 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNCIC1

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two 530 kDa heavy chains, and intermediate chains of ~74 kDa and 53-59 kDa.

Alternate Names

cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, DNCI1cytoplasmic, intermediate polypeptide 1, DNCIC1DH IC-1, Dynein intermediate chain 1, cytosolic, dynein, cytoplasmic 1, intermediate chain 1

Entrez Gene IDs

1780 (Human)

Gene Symbol

DYNC1I1

UniProt

O14576 (Human)

Additional DNCIC1 Products

Product Documents for DNCIC1 Antibody (74.1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNCIC1 Antibody (74.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DNCIC1 Antibody (74.1) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human colon tissue
    Species: Human
    Verified Customer | Posted 03/25/2015
    DNCIC1 in colon epithelial cells
    DNCIC1 Antibody (74.1) NB300-726
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Caco-2 cell line
    Species: Human
    Verified Customer | Posted 03/23/2015
    DYNC1I1 - IF
    DNCIC1 Antibody (74.1) NB300-726
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human colon cancer cell lysates
    Species: Human
    Verified Customer | Posted 03/23/2015
    DYNC1I1
    DNCIC1 Antibody (74.1) NB300-726

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Protocols

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FAQs for DNCIC1 Antibody (74.1)

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  • Q: Is the DNCIC1 antibody with the number NB300-726 is only specific for DNCIC1 protein? Does it detect the DNCIC2 protein as well?

    A: For our product NB300-726, we have not yet tested this product for cross reactivity against the DNCIC2 protein. These two proteins share quite a few regions of 100% homology, so I think it is highly likely that this product would cross react with the DNCIC2 protein.

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