Donor Horse Serum, Heat Inactivated Summary
Why Use Donor Horse Serum, Heat Inactivated?
Donor Horse Serum (DHS) is derived from healthy, live animals greater than 12 months of age. The donor animals receive regular veterinary inspection and care, and are kept in carefully managed and strictly segregated herds. The donor animals are all gelded draft horses in order to limit lot-to-lot hormone level variations A variety of cell types can be successfully propagated in DHS. It is frequently used in combination with bovine sera or independently to supplement media for mammalian cell culture. DHS is often utilized in bovine virus research applications since it does not contain bovine antibodies. It is also used as a supplement in some bacteriological media. Donor Horse Serum, Heat Inactivated is treated by heat inactivation where serum is exposed to a temperature of 56 °C for 30 minutes under controlled conditions.
Why Heat Inactivate Serum?
The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.
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