Dopa Decarboxylase/DDC Antibody - Azide Free
Novus Biologicals | Catalog # NB300-174
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine
Cited:
Human, Mouse, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
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Product Specifications
Immunogen
SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies. Accession # P27718
Reactivity Notes
Porcine reactivity reported in (PMID: 19857468).
Specificity
Specific for endogenous levels of the ~55 kDa Dopa Decarboxylase/DDC protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dopa Decarboxylase/DDC Antibody - Azide Free
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174]
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174]
Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.Western Blot: Dopa Decarboxylase/DDC Antibody - Azide Free [NB300-174] -
Expression levels of monoamine-regulating enzymes in the brain of SMN delta 7 mice.a, h, o Representative autoradiograms of brain lysates immunoblots of SMN delta 7 and wild-type (WT) mice at a post-natal day 3, h day 6, and o day 11. b–g Protein levels quantification of b Tyrosine hydroxylase (TH), c phospho-Tyrosine hydroxylase at Ser-40 (P-Ser40-TH), d Dopamine beta hydroxylase (D beta H), e Tryptophan hydroxylase 2 (TPH2), f Aromatic amino acid decarboxylase (AADC) and g Monoamine Oxidase A (MAO-A) in SMN delta 7 (n = 4) and WT (n = 5) mice (except for TPH2: n = 4 mice/genotype) at post-natal day 3. i–n Protein levels quantification of i TH (n = 4 WT, n = 5 SMN delta 7), j P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), k D beta H (n = 4 WT, n = 4 SMN delta 7), l TPH2 (n = 4 WT, n = 3 SMN delta 7), m AADC (n = 4 WT, n = 5 SMN delta 7) and n MAO-A (n = 4 WT, n = 5 SMN delta 7) at post-natal day 6. p–u Protein levels quantification of p TH (n = 13 WT, n = 14 SMN delta 7), q P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), r D beta H (n = 4 WT, n = 5 SMN delta 7), s TPH2 (n = 4 WT, n = 5 SMN delta 7), t AADC (n = 13 WT, n = 14 SMN delta 7) and u MAO-A (n = 8 WT, n = 10 SMN delta 7) at post-natal day 11. Data are normalized to beta -Actin levels and shown as box and whisker plots representing the median with interquartile range (IQR). Dots represent individual mice values. *p < 0.05, **p < 0.01, compared with age-matched WT mice (unpaired t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37957344), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Dopa Decarboxylase/DDC Antibody - Azide Free [NB300-174] -
Expression levels of monoamine-regulating enzymes in the brain of SMN delta 7 mice.a, h, o Representative autoradiograms of brain lysates immunoblots of SMN delta 7 and wild-type (WT) mice at a post-natal day 3, h day 6, and o day 11. b–g Protein levels quantification of b Tyrosine hydroxylase (TH), c phospho-Tyrosine hydroxylase at Ser-40 (P-Ser40-TH), d Dopamine beta hydroxylase (D beta H), e Tryptophan hydroxylase 2 (TPH2), f Aromatic amino acid decarboxylase (AADC) and g Monoamine Oxidase A (MAO-A) in SMN delta 7 (n = 4) and WT (n = 5) mice (except for TPH2: n = 4 mice/genotype) at post-natal day 3. i–n Protein levels quantification of i TH (n = 4 WT, n = 5 SMN delta 7), j P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), k D beta H (n = 4 WT, n = 4 SMN delta 7), l TPH2 (n = 4 WT, n = 3 SMN delta 7), m AADC (n = 4 WT, n = 5 SMN delta 7) and n MAO-A (n = 4 WT, n = 5 SMN delta 7) at post-natal day 6. p–u Protein levels quantification of p TH (n = 13 WT, n = 14 SMN delta 7), q P-Ser40-TH (n = 4 WT, n = 5 SMN delta 7), r D beta H (n = 4 WT, n = 5 SMN delta 7), s TPH2 (n = 4 WT, n = 5 SMN delta 7), t AADC (n = 13 WT, n = 14 SMN delta 7) and u MAO-A (n = 8 WT, n = 10 SMN delta 7) at post-natal day 11. Data are normalized to beta -Actin levels and shown as box and whisker plots representing the median with interquartile range (IQR). Dots represent individual mice values. *p < 0.05, **p < 0.01, compared with age-matched WT mice (unpaired t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37957344), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Dopa Decarboxylase/DDC Antibody - Azide Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Simple Western
1:500
Western Blot
1:1000
Application Notes
This antibody can be used in Western blot where a single band at 55 kDa is seen. Immunohistochemistry-Paraffin was reported in scientific literature.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Cerebellum lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:500. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse Cerebellum lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:500. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Format
Azide Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Dopa Decarboxylase/DDC
Alternate Names
AADC, DDC
Gene Symbol
DDC
UniProt
Additional Dopa Decarboxylase/DDC Products
Product Documents for Dopa Decarboxylase/DDC Antibody - Azide Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Dopa Decarboxylase/DDC Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Dopa Decarboxylase/DDC Antibody - Azide Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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