Dopa Decarboxylase/DDC Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-56918

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Synthetic peptides corresponding to DDC (dopa decarboxylase, aromatic L-amino acid decarboxylase). The peptide sequence was selected from the N terminal of DDC (NP_000781). Peptide sequence EFRRRGKEMVDYVANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29789702).

Localization

Cytosol

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Dopa Decarboxylase/DDC Antibody - BSA Free

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Reccomended Titration: 1 ug/ml Positive Control: Human 293T cell lysate
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - HepG2 cell lysate, Antibody Titration: 5.0ug/ml

Applications for Dopa Decarboxylase/DDC Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopa Decarboxylase/DDC

DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Alternate Names

AADC, DDC

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

UniProt

Additional Dopa Decarboxylase/DDC Products

Product Documents for Dopa Decarboxylase/DDC Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Dopa Decarboxylase/DDC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dopa Decarboxylase/DDC Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Dopa Decarboxylase/DDC Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All
  • Q: I would like to use your anti-DOPA Decarboxylase Antibody (NBP1-56918) in fixed mouse brain sections (IHC) and was wondering if it has been evaluated for this particular use? Do you also offer the DOPA Decarboxylase Peptide?

    A: In our validation studies, NBP1-56918 was tested with human kidney tissue in IHC-P application wherein this antibody showed nice staining at the antibody concentration range of 4-8ug/ml. Unfortunately, I do not have any data to provide on the mouse brain's IHC-P staining specifically at the moment, however, you should not be concerned because the immunogen sequence is 100% identical to mouse and we have validated this product successfully in IHC-P. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products. We do sell Blocking Peptide specific for DOPA Decarboxylase Antibody (NBP1-56918) and the catalog # for the peptide is NBP1-56918PEP.

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