Doublecortin Antibody (BLR168J)

Novus Biologicals | Catalog # NBP3-14742

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR168J
View all Doublecortin/DCX Primary Antibodies »

Product Specifications

Immunogen

between 200 and 250

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Doublecortin Antibody (BLR168J)

Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742]

Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742]

Western Blot: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunocytochemistry: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate DAB
Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB
Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742]

Immunohistochemistry-Paraffin: Doublecortin Antibody (BLR168J) [NBP3-14742] - Rabbit anti-Doublecortin recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate DAB
Doublecortin Antibody (BLR168J)

Immunoprecipitation- Doublecortin Antibody (BLR168J)-NBP3-14742-

Immunoprecipitation- Doublecortin Antibody (BLR168J)-NBP3-14742-Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit antiDoublecortin recombinant monoclonal antibody [BLR168J] used for IP at 20 ul/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin (BL-1974A-2B1). For blotting immunoprecipitated Doublecortin was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.

Applications for Doublecortin Antibody (BLR168J)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

20 µl/1 mg lysate

Western Blot

1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Doublecortin/DCX

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Gene Symbol

DCX

Additional Doublecortin/DCX Products

Product Documents for Doublecortin Antibody (BLR168J)

Certificate of Analysis

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Product Specific Notices for Doublecortin Antibody (BLR168J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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