Doublecortin Antibody

Name: Doublecortin Antibody
Brand: Novus Biologicals
SKU: NBP2-26149
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-26149

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Doublecortin/DCX Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PKASPTPQKTSAKS corresponding to internal region according to NP_001103694.1, NP_034155.2.

Reactivity Notes

Expected from sequence similarity: Rat

Marker

Immature Neuronal Marker

Specificity

This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Doublecortin Antibody

Western Blot: Doublecortin Antibody [NBP2-26149]

Western Blot: Doublecortin Antibody [NBP2-26149] - NBP2-26149 (0.5ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP2-26149]

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP2-26149] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml)

Applications for Doublecortin Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml

Application Notes

WB: Approx. 40 kDa band observed in mouse fetal brain lysates (calculated MW of 40.6 kDa band according to NP_001103692.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Doublecortin/DCX

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Entrez Gene IDs

1641 (Human); 13193 (Mouse); 84394 (Rat)

Gene Symbol

DCX

Additional Doublecortin/DCX Products

Product Documents for Doublecortin Antibody

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Certificate of Analysis

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Product Specific Notices for Doublecortin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

Neural Progenitor Cell Markers

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