DPYD Antibody

Novus Biologicals | Catalog # NBP3-48502

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DPYD Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DPYD. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DPYD Antibody

DPYD Antibody

Immunohistochemistry: DPYD Antibody [NBP3-48502] -

DPYD antibody [N1N2], N-term detects DPYD protein at cytoplasm on H358 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H358 xenograft.
DPYD antibody [N1N2], N-term (NBP3-48502) dilution: 1:500.


Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
DPYD Antibody

Western Blot: DPYD Antibody [NBP3-48502] -

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (NBP3-48502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DPYD Antibody

Western Blot: DPYD Antibody [NBP3-48502] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (NBP3-48502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for DPYD Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPYD

DPYD is encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy.

Alternate Names

DHP, DHPDHASE, dihydropyrimidine dehydrogenase, dihydropyrimidine dehydrogenase [NADP+], Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPDMGC132008, EC 1.3.1.2, MGC70799

Gene Symbol

DPYD

Additional DPYD Products

Product Documents for DPYD Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for DPYD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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