Drebrin 1 Antibody (DBN1/2880)

Novus Biologicals | Catalog # NBP2-79767

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DBN1/2880
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Product Specifications

Immunogen

Recombinant fragment (aa 150-281) of human Drebrin 1 protein (exact sequence is proprietary) (Uniprot: Q16643)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79902)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Drebrin 1 Antibody (DBN1/2880)

Western Blot: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Western Blot: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Western Blot: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature. Image using the Alexa Fluor 647 format of this antibody.
Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767] - Formalin-fixed, paraffin-embedded human Cervix stained with Drebrin 1 Antibody (DBN1/2880).
Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Immunohistochemistry-Paraffin: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767] - Formalin-fixed, paraffin-embedded human Brain stained with Drebrin 1 Antibody (DBN1/2880).
Protein Array: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Protein Array: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767]

Protein Array: Drebrin 1 Antibody (DBN1/2880) [NBP2-79767] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Drebrin 1 Antibody (DBN1/2880). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Drebrin 1 Antibody (DBN1/2880)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Drebrin 1

Drebrins are actin-binding proteins, that are developmentally regulated in the process of neuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. The expression pattern of each isoform is regulated spatially and temporally in the developing brain. It has been suggested that debrin is one of the intracellular regulators of the neuronal morphogenesis (4). Debrin has also been detected in other kinds of non-neuronal human and bovine tissue but its exact role is unknown.

Alternate Names

D0S117E, DBN1, Drba, Drebrin A, Drebrin E, Drebrin E2

Gene Symbol

DBN1

UniProt

Additional Drebrin 1 Products

Product Documents for Drebrin 1 Antibody (DBN1/2880)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Drebrin 1 Antibody (DBN1/2880)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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